Optimization of Genomic DNA Isolation in Date Palm (Phoenix dactylifera L.) for Molecular Analysis.
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Abstract
Molecular marker techniques are essential for cultivar identification and biodiversity conservation of date palms (Phoenix dactylifera L.). High-purity genomic DNA is a prerequisite for PCR amplification, DNA fingerprinting, and sequencing in molecular breeding. This study evaluates the effectiveness of the cetyltrimethylammonium bromide (CTAB) method in achieving high-quality DNA yields suitable for PCR applications. Additionally, a comparative analysis with other existing DNA extraction methods, including silica-based column purification and magnetic bead-based extraction, is presented to highlight the advantages and limitations of each approach. The results demonstrate that the inclusion of sodium chloride (NaCl) (1.4 M) in the lysis buffer, coupled with mechanical grinding using liquid nitrogen and sterile sand, enhances DNA purity without the need for polyvinylpyrrolidone (PVP) or lithium chloride (LiCl). Comparisons with alternative DNA isolation methods highlight the cost-effectiveness and efficiency of this approach. These findings suggest a viable alternative for DNA isolation in date palms, with significant implications for molecular breeding and conservation research
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